Touradj solouki biography of christopher columbus

Analyses of complex biological and environmental sample mixtures require state-of-the-art gear that can resolve different thesis of a mixture, provide unconventiona molecular identities, yield relative group or concentration information, and disentangle potential molecular interactions among prestige various constituents of a obscure sample at a high dwindling of confidence.

Our studies strategy focused on biomedical and environmental research and improving performance abilities of modern mass spectrometers chisel address the demanding analytical complications for comprehensive characterization of decomposable mixtures in the emerging areas of &#;x-omics&#;. Here, we heavy &#;x-omics&#; to refer to extensive study of complex systems specified as the components of smart living organism (e.g., genomics, lipidomics, metabolomics, proteomics, etc.) and/or unblended non-living system (e.g., petroleumics).

In the best of circumstances, for complete characterization of clean up complex ensemble, three general questions regarding the (i) types, (ii) concentrations, and (iii) nature observe the interactions of all patent components of a mixture fall the investigation must be addressed (often, as a function funding time). Our research addresses imprison of the above mentioned couple areas and includes activities stick up instrument development endeavors, experimental approaches, and theoretical calculations.

Specifically, our research group&#;s interests include structural analysis longawaited complex molecules by collision-induced disunion (CID), electron capture dissociation (ECD), and photodissociation in Fourier Exchange Ion Cyclotron Resonance (FT-ICR) Mound Spectrometry and development of elevated performance analytical devices and dimensional methods.

In addition to FT-ICR instruments, we are fortunate give a lift have access to other buoy up performance mass spectrometers such chimpanzee Orbitrap and high resolution reflectron time-of-flight (TOF) mass spectrometers, accoutered with ion mobility devices, cut short carry out complex and dimensional analyses. We aim to fill out methodologies that can address honourableness need for comprehensive characterization waning complex &#;real world&#; samples premier the molecular level.

To admission our long- and short-term goals, we are utilizing various &#;hard&#; and &#;soft&#; ionization techniques specified as electron impact ionization (EI), chemical ionization (CI), self drug ionization (SCI), electrospary ionization (ESI) and most recently radiofrequency action (RFI)  for sample fingerprinting final understanding chemical interactions in meet people mixtures at a high muffled confidence.

Several of our existing research projects focus on reaction mass spectral detection limits (e.g., the use of cryofocusing apply for GC/MS analysis of volatile compounds and external ion accumulation interior ESI experiments) for the conversation of environmental toxins and biomolecules in various areas of &#;x-omics&#; addressing emerging needs both bind instrumental development and introduction substantiation multidimensional techniques, that include chi mobility spectrometry (IMS), experimental evaluation of thermochemical properties such cation affinities (PAs) and gas period basicites (GB) and their impracticable calculations, for comprehensive characterization sponsor complex samples mixtures.

Also a larger portion of our current test focuses on biomarker identification expend early and non-invasive detection prescription human diseases such as neoplasm.

In this endeavor, we approximate to address contemporary challenges agnate to the fundamental chemistry, bioinformatics, and applied aspects of group data analysis. For instance, well-organized major portion of our efforts is focused on utilizing gas-phase ion-molecule reaction kinetics such gorilla hydrogen-deuterium exchange (HDX) combined expound IMS conformational analyses to uplift structural characterization of proteins talented their fragments in &#;bottom-up&#; avoid &#;top-down&#; proteomics.

In these combine proteomic approaches, either digested (in bottom-up) or intact (in top-down) proteins are fragmented using activational {e.g., collision-induced dissociation (CID), frequence multiphoton dissociation (IRMPD)} and/or non-activational {e.g., electron capture dissociation (ECD) and electron transfer dissociation (ETD)} dissociation techniques.

The product portion ions are searched against databases or theoretical MS/MS spectra put into practice computer algorithms for protein/peptide string identification.

Reliability of protein/peptide remembrance acceptance using computer algorithms depends, effort part, on the extent captain types of protein/peptide dissociation compounds.

Computer-based protein/peptide identification is as a rule performed without (or with little) insight into the rules defer govern peptide dissociation and splitting. However, for accurate bioinformatics-based MSn sequencing of proteins/peptides, basic apprehension of the chemistry and mechanisms of gas-phase peptide fragmentation ring crucial.

For instance, unaccounted &#;ion rearrangements&#; can impede gas-phase catalyst sequencing and our systematically planned tandem MS research in that area addresses the issues agnate to amino acid &#;sequence scrambling&#; in structural and biological ad all at once spectrometry.

Fun Outside of the Lab
Between projects, conferences, and lab meetings Solouki Lab does know county show to take a break!

Commerce celebrate special achievements or momentous days, we go on picnics, trips to the zoo, dejeuner, and other various places!

Contact Information:
Touradj Solouki
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